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trizol reagent  (Thermo Fisher)


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    Structured Review

    Thermo Fisher trizol reagent
    Trizol Reagent, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/trizol+reagent/Phosphate/pmc13092056-124-11-13
    Average 99 stars, based on 1 article reviews
    trizol reagent - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Isolation:

    Article Title: Zwitterionic hydrogel designs for conducting polymers enable bioelectronics with suppressed foreign body responses
    Article Snippet: .. For RNA isolation, the tissues were thawed, finely diced, and 600 μL of Trizol reagent (Invitrogen) with 10 μL/mL of 2-mercaptoethanol was added to approximately 100 mg of tissue. ..

    Article Title: Translational regulation of PKD1 by evolutionarily conserved upstream open reading frames.
    Article Snippet: .. Total RNA was isolated with the TRIzol Reagent (Life Technologies) following the manufacturer’s instructions. .. The concentration of RNA was quantified using UV-Vis spectrophotometer Q5000 (Quawell). cDNA synthesis and qPCR were performed with the Luna Universal One-Step RTqPCR Kit (NEB) on the 7900HT Fast Real-Time PCR System (Applied Biosystems) according to the manufacturer’s guideline.

    Saline:

    Article Title: Revolutionizing Mg-based guided bone regeneration mesh derived from endogenous dentoalveolar bone augmentation
    Article Snippet: .. The cells were rinsed twice with DPBS (Dulbecco's Phosphate-Buffered Saline), then TRIzol reagent (Thermo Fisher Scientific, USA) was added. .. RNA extraction, transcriptome sequencing, and subsequent analysis were performed by Shanghai OE Biotech Co., Ltd. RNA purity and quantification were assessed using a NanoDrop 2000 (Thermo Scientific, USA).

    Article Title: NSC777201 exhibits anticancer activity against colorectal cancer cells by inducing multiple types of cell death.
    Article Snippet: McCoy’s 5A medium (10-050-CVS) and fetal bovine serum (FBS; 35-010-CV) were purchased from Corning (Corning, NY, USA). .. Roswell Park Memorial Institute (RPMI)-1640 medium (22400071), L-glutamine (25030-81), non-essential amino acids (NEAAs; 11140050), sodium pyruvate (11360070), an antibiotic-antimycotic solution (PSA contains 10,000 units/mL penicillin, 10,000 μg/mL streptomycin, and 25 μg/mL of amphotericin B; 15240-062), phosphate-buffered saline (PBS) tablets (18912014), radioimmunoprecipitation assay (RIPA) lysis and extraction buffer (89901), and TRIzol reagent (15596026) were purchased from Thermo Fisher Scientific (Waltham, MA, USA). .. Chloroquine (C6628), Spautin-1 (SML0440), deferoxamine (D9533), dimethyl sulfoxide (DMSO; D5879), phosphatase inhibitor cocktail tablets (04906837001), and protease inhibitor cocktail tablets (11873580001) were purchased from Sigma-Aldrich (Munich, Germany).

    Reverse Transcription:

    Article Title: Hesperidin alleviates pulmonary fibrosis by regulating EI24-mediated autophagy.
    Article Snippet: .. Reverse transcription quantitative polymerase chain reaction (RT-qPCR) total RNa was extracted from Rle-6tN cells using tRizol reagent (#15596026, thermo Fisher scientific, Waltham, Ma, usa) and then executed with reversal transcription using the Bio-Rad scriptM cDNa synthesis Kit (#1708890, Bio-Rad laboratories, inc., hercules, ca, usa). .. Rt-qPcR was performed on the Bio-Rad cFX Manager software (Bio-Rad laboratories, inc.) with 2 × sYBR Master mix (#RR820a, takara, Dalian, china). the Rt-qPcR conditions were 5 min at 94 °c, followed by 40 cycles between 94 °c for 10 sec and 58 °c for 45 sec, and 72 °c for 1 min. the expression levels of genes were determined by the 2-ΔΔct method, with the GAPDH as the internal reference gene. the primers were designed and synthesized in sangon Biotech (shanghai, china). the primer sequences were as follows: 5′-tGGccactGctGcttcctcttctt-3′ (α-SMA forward), 5′-GGGGccaGcttcGtc atactcct-3′ (α-SMA reverse); 5′-tGttGGtcctGctGGcaaGaatG-3′ (collagen I forward), 5′-GtcaccttGttcGcct Gtctcac-3′ (collagen I reverse); 5′-ataaatcaGcaGtGGGaacG-3′ (fibronectin forward), 5′-cttGtcGccaatctt GtaGG-3′ (fibronectin reverse); 5′-cGGcaaGttcaacGGcacaGtca-3′ (GAPDH forward), 5′-ccacGacatactcaGca ccaGcatca-3′ (GAPDH reverse).

    Article Title: Lactylation of β-catenin promotes non-small cell lung cancer by inhibiting ferroptosis through TCF4-mediated GPX4 transcription.
    Article Snippet: 2.1 Antibodies and Reagents Antibodies: β-catenin antibody (ABclonal, China), Pan-Kla antibody (ABclonal, China, Cat. No.: A23004), β-catenin-Kla antibody (custom site-specific antibody, Beinlai Biotechnology Co., Ltd.), GPX4 antibody (ABclonal, China), GAPDH antibody (ABclonal, China), goat anti-rabbit IgG-HRP (ABclonal, China), goat anti-mouse IgGHRP (ABclonal, China). .. Reagents: Fetal bovine serum (FBS, Gibco, USA), DMEM medium (Gibco, USA), RPMI-1640 medium (Gibco, USA), PBS buffer (Kaiji Biology, China), trypsin (Gibco, USA), cryopreservation solution (Saiwen Innovation, China), paraformaldehyde (Solarbio, China), 10×TBST buffer (Solarbio, China), Triton X-100 (Solarbio, China), Trizol reagent (Invitrogen, USA), isopropanol (Sinopharm, China), absolute ethanol (Shanghai Bio-Chemical Co., Ltd., China), TB Green Premix Ex Taq II (Takara, Japan), reverse transcription kit (Takara, Japan), Puromycin dihydrochloride (MCE, USA), PCR primers (Sangon Biotech, China), overexpression virus (Sangon Biotech, China), knockdown virus (Sangon Biotech, China), CCK-8 kit (Solarbio, China), Matrigel Matrix (Corning, USA), EdU cell proliferation assay kit (Solarbio, China), protein AR TIC LE IN PR ES S extraction kit (Kaiji Biology, China), BCA protein assay kit (Beyotime, China), SDSPAGE gel preparation kit (Kaiji Biology, China), PVDF membrane (Millipore, USA), ECL chemiluminescence reagent (Tanon, China), reduced glutathione (GSH) assay kit (Solarbio, China), lipid peroxide (LPO) assay kit (Solarbio, China). lactic acid (LA, Sigma-Aldrich, USA), 2-deoxyglucose (2-DG, Sigma-Aldrich, USA), Fer-1 (Abcam, UK), Beclin 1Bioswamp, China. ..

    Real-time Polymerase Chain Reaction:

    Article Title: Hesperidin alleviates pulmonary fibrosis by regulating EI24-mediated autophagy.
    Article Snippet: .. Reverse transcription quantitative polymerase chain reaction (RT-qPCR) total RNa was extracted from Rle-6tN cells using tRizol reagent (#15596026, thermo Fisher scientific, Waltham, Ma, usa) and then executed with reversal transcription using the Bio-Rad scriptM cDNa synthesis Kit (#1708890, Bio-Rad laboratories, inc., hercules, ca, usa). .. Rt-qPcR was performed on the Bio-Rad cFX Manager software (Bio-Rad laboratories, inc.) with 2 × sYBR Master mix (#RR820a, takara, Dalian, china). the Rt-qPcR conditions were 5 min at 94 °c, followed by 40 cycles between 94 °c for 10 sec and 58 °c for 45 sec, and 72 °c for 1 min. the expression levels of genes were determined by the 2-ΔΔct method, with the GAPDH as the internal reference gene. the primers were designed and synthesized in sangon Biotech (shanghai, china). the primer sequences were as follows: 5′-tGGccactGctGcttcctcttctt-3′ (α-SMA forward), 5′-GGGGccaGcttcGtc atactcct-3′ (α-SMA reverse); 5′-tGttGGtcctGctGGcaaGaatG-3′ (collagen I forward), 5′-GtcaccttGttcGcct Gtctcac-3′ (collagen I reverse); 5′-ataaatcaGcaGtGGGaacG-3′ (fibronectin forward), 5′-cttGtcGccaatctt GtaGG-3′ (fibronectin reverse); 5′-cGGcaaGttcaacGGcacaGtca-3′ (GAPDH forward), 5′-ccacGacatactcaGca ccaGcatca-3′ (GAPDH reverse).

    cDNA Synthesis:

    Article Title: Hesperidin alleviates pulmonary fibrosis by regulating EI24-mediated autophagy.
    Article Snippet: .. Reverse transcription quantitative polymerase chain reaction (RT-qPCR) total RNa was extracted from Rle-6tN cells using tRizol reagent (#15596026, thermo Fisher scientific, Waltham, Ma, usa) and then executed with reversal transcription using the Bio-Rad scriptM cDNa synthesis Kit (#1708890, Bio-Rad laboratories, inc., hercules, ca, usa). .. Rt-qPcR was performed on the Bio-Rad cFX Manager software (Bio-Rad laboratories, inc.) with 2 × sYBR Master mix (#RR820a, takara, Dalian, china). the Rt-qPcR conditions were 5 min at 94 °c, followed by 40 cycles between 94 °c for 10 sec and 58 °c for 45 sec, and 72 °c for 1 min. the expression levels of genes were determined by the 2-ΔΔct method, with the GAPDH as the internal reference gene. the primers were designed and synthesized in sangon Biotech (shanghai, china). the primer sequences were as follows: 5′-tGGccactGctGcttcctcttctt-3′ (α-SMA forward), 5′-GGGGccaGcttcGtc atactcct-3′ (α-SMA reverse); 5′-tGttGGtcctGctGGcaaGaatG-3′ (collagen I forward), 5′-GtcaccttGttcGcct Gtctcac-3′ (collagen I reverse); 5′-ataaatcaGcaGtGGGaacG-3′ (fibronectin forward), 5′-cttGtcGccaatctt GtaGG-3′ (fibronectin reverse); 5′-cGGcaaGttcaacGGcacaGtca-3′ (GAPDH forward), 5′-ccacGacatactcaGca ccaGcatca-3′ (GAPDH reverse).

    RNA Sequencing:

    Article Title: Deciphering the biosynthetic pathways of lichen acids.
    Article Snippet: .. For RNA-seq analysis, total RNA was extracted from 100 mg of the ground samples using TRIzol reagent (ThermoFisher Scientific), according to the manufacturer’s instructions with additional extraction steps: a phenol (pH 4.6)–chloroform– isoamyl alcohol (25 : 24 : 1) extraction step followed by a chloroform extraction step after the initial TRIzol-chloroform phase separation. .. RNA pellets were dissolved in 88 ll of nuclease-free water and treated with RNase-Free DNase set (Qiagen, Hilden, Germany) to remove possible genomic DNA contamination.

    Extraction:

    Article Title: Deciphering the biosynthetic pathways of lichen acids.
    Article Snippet: .. For RNA-seq analysis, total RNA was extracted from 100 mg of the ground samples using TRIzol reagent (ThermoFisher Scientific), according to the manufacturer’s instructions with additional extraction steps: a phenol (pH 4.6)–chloroform– isoamyl alcohol (25 : 24 : 1) extraction step followed by a chloroform extraction step after the initial TRIzol-chloroform phase separation. .. RNA pellets were dissolved in 88 ll of nuclease-free water and treated with RNase-Free DNase set (Qiagen, Hilden, Germany) to remove possible genomic DNA contamination.

    Article Title: NSC777201 exhibits anticancer activity against colorectal cancer cells by inducing multiple types of cell death.
    Article Snippet: McCoy’s 5A medium (10-050-CVS) and fetal bovine serum (FBS; 35-010-CV) were purchased from Corning (Corning, NY, USA). .. Roswell Park Memorial Institute (RPMI)-1640 medium (22400071), L-glutamine (25030-81), non-essential amino acids (NEAAs; 11140050), sodium pyruvate (11360070), an antibiotic-antimycotic solution (PSA contains 10,000 units/mL penicillin, 10,000 μg/mL streptomycin, and 25 μg/mL of amphotericin B; 15240-062), phosphate-buffered saline (PBS) tablets (18912014), radioimmunoprecipitation assay (RIPA) lysis and extraction buffer (89901), and TRIzol reagent (15596026) were purchased from Thermo Fisher Scientific (Waltham, MA, USA). .. Chloroquine (C6628), Spautin-1 (SML0440), deferoxamine (D9533), dimethyl sulfoxide (DMSO; D5879), phosphatase inhibitor cocktail tablets (04906837001), and protease inhibitor cocktail tablets (11873580001) were purchased from Sigma-Aldrich (Munich, Germany).

    Article Title: Lactylation of β-catenin promotes non-small cell lung cancer by inhibiting ferroptosis through TCF4-mediated GPX4 transcription.
    Article Snippet: 2.1 Antibodies and Reagents Antibodies: β-catenin antibody (ABclonal, China), Pan-Kla antibody (ABclonal, China, Cat. No.: A23004), β-catenin-Kla antibody (custom site-specific antibody, Beinlai Biotechnology Co., Ltd.), GPX4 antibody (ABclonal, China), GAPDH antibody (ABclonal, China), goat anti-rabbit IgG-HRP (ABclonal, China), goat anti-mouse IgGHRP (ABclonal, China). .. Reagents: Fetal bovine serum (FBS, Gibco, USA), DMEM medium (Gibco, USA), RPMI-1640 medium (Gibco, USA), PBS buffer (Kaiji Biology, China), trypsin (Gibco, USA), cryopreservation solution (Saiwen Innovation, China), paraformaldehyde (Solarbio, China), 10×TBST buffer (Solarbio, China), Triton X-100 (Solarbio, China), Trizol reagent (Invitrogen, USA), isopropanol (Sinopharm, China), absolute ethanol (Shanghai Bio-Chemical Co., Ltd., China), TB Green Premix Ex Taq II (Takara, Japan), reverse transcription kit (Takara, Japan), Puromycin dihydrochloride (MCE, USA), PCR primers (Sangon Biotech, China), overexpression virus (Sangon Biotech, China), knockdown virus (Sangon Biotech, China), CCK-8 kit (Solarbio, China), Matrigel Matrix (Corning, USA), EdU cell proliferation assay kit (Solarbio, China), protein AR TIC LE IN PR ES S extraction kit (Kaiji Biology, China), BCA protein assay kit (Beyotime, China), SDSPAGE gel preparation kit (Kaiji Biology, China), PVDF membrane (Millipore, USA), ECL chemiluminescence reagent (Tanon, China), reduced glutathione (GSH) assay kit (Solarbio, China), lipid peroxide (LPO) assay kit (Solarbio, China). lactic acid (LA, Sigma-Aldrich, USA), 2-deoxyglucose (2-DG, Sigma-Aldrich, USA), Fer-1 (Abcam, UK), Beclin 1Bioswamp, China. ..

    RNA Extraction:

    Article Title: NLRP1 as a Novel Pyroptosis Biomarker in Irreversible Pulpitis: A Laboratory Investigation and Animal Model Study.
    Article Snippet: Aim: Irreversible pulpitis poses a significant clinical burden due to progressive inflammatory pulp damage.. While inflammatory mechanisms are central to its pathogenesis, they remain incompletely characterised.. This study aims to elucidate the role of Nodlike receptor thermal domain associated protein 1 (NLRP1) in irreversible pulpitis pathogenesis.

    Protein Extraction:

    Article Title: NLRP1 as a Novel Pyroptosis Biomarker in Irreversible Pulpitis: A Laboratory Investigation and Animal Model Study.
    Article Snippet: Aim: Irreversible pulpitis poses a significant clinical burden due to progressive inflammatory pulp damage.. While inflammatory mechanisms are central to its pathogenesis, they remain incompletely characterised.. This study aims to elucidate the role of Nodlike receptor thermal domain associated protein 1 (NLRP1) in irreversible pulpitis pathogenesis.

    Radio Immunoprecipitation:

    Article Title: NSC777201 exhibits anticancer activity against colorectal cancer cells by inducing multiple types of cell death.
    Article Snippet: McCoy’s 5A medium (10-050-CVS) and fetal bovine serum (FBS; 35-010-CV) were purchased from Corning (Corning, NY, USA). .. Roswell Park Memorial Institute (RPMI)-1640 medium (22400071), L-glutamine (25030-81), non-essential amino acids (NEAAs; 11140050), sodium pyruvate (11360070), an antibiotic-antimycotic solution (PSA contains 10,000 units/mL penicillin, 10,000 μg/mL streptomycin, and 25 μg/mL of amphotericin B; 15240-062), phosphate-buffered saline (PBS) tablets (18912014), radioimmunoprecipitation assay (RIPA) lysis and extraction buffer (89901), and TRIzol reagent (15596026) were purchased from Thermo Fisher Scientific (Waltham, MA, USA). .. Chloroquine (C6628), Spautin-1 (SML0440), deferoxamine (D9533), dimethyl sulfoxide (DMSO; D5879), phosphatase inhibitor cocktail tablets (04906837001), and protease inhibitor cocktail tablets (11873580001) were purchased from Sigma-Aldrich (Munich, Germany).

    Lysis:

    Article Title: NSC777201 exhibits anticancer activity against colorectal cancer cells by inducing multiple types of cell death.
    Article Snippet: McCoy’s 5A medium (10-050-CVS) and fetal bovine serum (FBS; 35-010-CV) were purchased from Corning (Corning, NY, USA). .. Roswell Park Memorial Institute (RPMI)-1640 medium (22400071), L-glutamine (25030-81), non-essential amino acids (NEAAs; 11140050), sodium pyruvate (11360070), an antibiotic-antimycotic solution (PSA contains 10,000 units/mL penicillin, 10,000 μg/mL streptomycin, and 25 μg/mL of amphotericin B; 15240-062), phosphate-buffered saline (PBS) tablets (18912014), radioimmunoprecipitation assay (RIPA) lysis and extraction buffer (89901), and TRIzol reagent (15596026) were purchased from Thermo Fisher Scientific (Waltham, MA, USA). .. Chloroquine (C6628), Spautin-1 (SML0440), deferoxamine (D9533), dimethyl sulfoxide (DMSO; D5879), phosphatase inhibitor cocktail tablets (04906837001), and protease inhibitor cocktail tablets (11873580001) were purchased from Sigma-Aldrich (Munich, Germany).

    Polymerase Chain Reaction:

    Article Title: Lactylation of β-catenin promotes non-small cell lung cancer by inhibiting ferroptosis through TCF4-mediated GPX4 transcription.
    Article Snippet: 2.1 Antibodies and Reagents Antibodies: β-catenin antibody (ABclonal, China), Pan-Kla antibody (ABclonal, China, Cat. No.: A23004), β-catenin-Kla antibody (custom site-specific antibody, Beinlai Biotechnology Co., Ltd.), GPX4 antibody (ABclonal, China), GAPDH antibody (ABclonal, China), goat anti-rabbit IgG-HRP (ABclonal, China), goat anti-mouse IgGHRP (ABclonal, China). .. Reagents: Fetal bovine serum (FBS, Gibco, USA), DMEM medium (Gibco, USA), RPMI-1640 medium (Gibco, USA), PBS buffer (Kaiji Biology, China), trypsin (Gibco, USA), cryopreservation solution (Saiwen Innovation, China), paraformaldehyde (Solarbio, China), 10×TBST buffer (Solarbio, China), Triton X-100 (Solarbio, China), Trizol reagent (Invitrogen, USA), isopropanol (Sinopharm, China), absolute ethanol (Shanghai Bio-Chemical Co., Ltd., China), TB Green Premix Ex Taq II (Takara, Japan), reverse transcription kit (Takara, Japan), Puromycin dihydrochloride (MCE, USA), PCR primers (Sangon Biotech, China), overexpression virus (Sangon Biotech, China), knockdown virus (Sangon Biotech, China), CCK-8 kit (Solarbio, China), Matrigel Matrix (Corning, USA), EdU cell proliferation assay kit (Solarbio, China), protein AR TIC LE IN PR ES S extraction kit (Kaiji Biology, China), BCA protein assay kit (Beyotime, China), SDSPAGE gel preparation kit (Kaiji Biology, China), PVDF membrane (Millipore, USA), ECL chemiluminescence reagent (Tanon, China), reduced glutathione (GSH) assay kit (Solarbio, China), lipid peroxide (LPO) assay kit (Solarbio, China). lactic acid (LA, Sigma-Aldrich, USA), 2-deoxyglucose (2-DG, Sigma-Aldrich, USA), Fer-1 (Abcam, UK), Beclin 1Bioswamp, China. ..

    Over Expression:

    Article Title: Lactylation of β-catenin promotes non-small cell lung cancer by inhibiting ferroptosis through TCF4-mediated GPX4 transcription.
    Article Snippet: 2.1 Antibodies and Reagents Antibodies: β-catenin antibody (ABclonal, China), Pan-Kla antibody (ABclonal, China, Cat. No.: A23004), β-catenin-Kla antibody (custom site-specific antibody, Beinlai Biotechnology Co., Ltd.), GPX4 antibody (ABclonal, China), GAPDH antibody (ABclonal, China), goat anti-rabbit IgG-HRP (ABclonal, China), goat anti-mouse IgGHRP (ABclonal, China). .. Reagents: Fetal bovine serum (FBS, Gibco, USA), DMEM medium (Gibco, USA), RPMI-1640 medium (Gibco, USA), PBS buffer (Kaiji Biology, China), trypsin (Gibco, USA), cryopreservation solution (Saiwen Innovation, China), paraformaldehyde (Solarbio, China), 10×TBST buffer (Solarbio, China), Triton X-100 (Solarbio, China), Trizol reagent (Invitrogen, USA), isopropanol (Sinopharm, China), absolute ethanol (Shanghai Bio-Chemical Co., Ltd., China), TB Green Premix Ex Taq II (Takara, Japan), reverse transcription kit (Takara, Japan), Puromycin dihydrochloride (MCE, USA), PCR primers (Sangon Biotech, China), overexpression virus (Sangon Biotech, China), knockdown virus (Sangon Biotech, China), CCK-8 kit (Solarbio, China), Matrigel Matrix (Corning, USA), EdU cell proliferation assay kit (Solarbio, China), protein AR TIC LE IN PR ES S extraction kit (Kaiji Biology, China), BCA protein assay kit (Beyotime, China), SDSPAGE gel preparation kit (Kaiji Biology, China), PVDF membrane (Millipore, USA), ECL chemiluminescence reagent (Tanon, China), reduced glutathione (GSH) assay kit (Solarbio, China), lipid peroxide (LPO) assay kit (Solarbio, China). lactic acid (LA, Sigma-Aldrich, USA), 2-deoxyglucose (2-DG, Sigma-Aldrich, USA), Fer-1 (Abcam, UK), Beclin 1Bioswamp, China. ..

    Virus:

    Article Title: Lactylation of β-catenin promotes non-small cell lung cancer by inhibiting ferroptosis through TCF4-mediated GPX4 transcription.
    Article Snippet: 2.1 Antibodies and Reagents Antibodies: β-catenin antibody (ABclonal, China), Pan-Kla antibody (ABclonal, China, Cat. No.: A23004), β-catenin-Kla antibody (custom site-specific antibody, Beinlai Biotechnology Co., Ltd.), GPX4 antibody (ABclonal, China), GAPDH antibody (ABclonal, China), goat anti-rabbit IgG-HRP (ABclonal, China), goat anti-mouse IgGHRP (ABclonal, China). .. Reagents: Fetal bovine serum (FBS, Gibco, USA), DMEM medium (Gibco, USA), RPMI-1640 medium (Gibco, USA), PBS buffer (Kaiji Biology, China), trypsin (Gibco, USA), cryopreservation solution (Saiwen Innovation, China), paraformaldehyde (Solarbio, China), 10×TBST buffer (Solarbio, China), Triton X-100 (Solarbio, China), Trizol reagent (Invitrogen, USA), isopropanol (Sinopharm, China), absolute ethanol (Shanghai Bio-Chemical Co., Ltd., China), TB Green Premix Ex Taq II (Takara, Japan), reverse transcription kit (Takara, Japan), Puromycin dihydrochloride (MCE, USA), PCR primers (Sangon Biotech, China), overexpression virus (Sangon Biotech, China), knockdown virus (Sangon Biotech, China), CCK-8 kit (Solarbio, China), Matrigel Matrix (Corning, USA), EdU cell proliferation assay kit (Solarbio, China), protein AR TIC LE IN PR ES S extraction kit (Kaiji Biology, China), BCA protein assay kit (Beyotime, China), SDSPAGE gel preparation kit (Kaiji Biology, China), PVDF membrane (Millipore, USA), ECL chemiluminescence reagent (Tanon, China), reduced glutathione (GSH) assay kit (Solarbio, China), lipid peroxide (LPO) assay kit (Solarbio, China). lactic acid (LA, Sigma-Aldrich, USA), 2-deoxyglucose (2-DG, Sigma-Aldrich, USA), Fer-1 (Abcam, UK), Beclin 1Bioswamp, China. ..

    Knockdown:

    Article Title: Lactylation of β-catenin promotes non-small cell lung cancer by inhibiting ferroptosis through TCF4-mediated GPX4 transcription.
    Article Snippet: 2.1 Antibodies and Reagents Antibodies: β-catenin antibody (ABclonal, China), Pan-Kla antibody (ABclonal, China, Cat. No.: A23004), β-catenin-Kla antibody (custom site-specific antibody, Beinlai Biotechnology Co., Ltd.), GPX4 antibody (ABclonal, China), GAPDH antibody (ABclonal, China), goat anti-rabbit IgG-HRP (ABclonal, China), goat anti-mouse IgGHRP (ABclonal, China). .. Reagents: Fetal bovine serum (FBS, Gibco, USA), DMEM medium (Gibco, USA), RPMI-1640 medium (Gibco, USA), PBS buffer (Kaiji Biology, China), trypsin (Gibco, USA), cryopreservation solution (Saiwen Innovation, China), paraformaldehyde (Solarbio, China), 10×TBST buffer (Solarbio, China), Triton X-100 (Solarbio, China), Trizol reagent (Invitrogen, USA), isopropanol (Sinopharm, China), absolute ethanol (Shanghai Bio-Chemical Co., Ltd., China), TB Green Premix Ex Taq II (Takara, Japan), reverse transcription kit (Takara, Japan), Puromycin dihydrochloride (MCE, USA), PCR primers (Sangon Biotech, China), overexpression virus (Sangon Biotech, China), knockdown virus (Sangon Biotech, China), CCK-8 kit (Solarbio, China), Matrigel Matrix (Corning, USA), EdU cell proliferation assay kit (Solarbio, China), protein AR TIC LE IN PR ES S extraction kit (Kaiji Biology, China), BCA protein assay kit (Beyotime, China), SDSPAGE gel preparation kit (Kaiji Biology, China), PVDF membrane (Millipore, USA), ECL chemiluminescence reagent (Tanon, China), reduced glutathione (GSH) assay kit (Solarbio, China), lipid peroxide (LPO) assay kit (Solarbio, China). lactic acid (LA, Sigma-Aldrich, USA), 2-deoxyglucose (2-DG, Sigma-Aldrich, USA), Fer-1 (Abcam, UK), Beclin 1Bioswamp, China. ..

    CCK-8 Assay:

    Article Title: Lactylation of β-catenin promotes non-small cell lung cancer by inhibiting ferroptosis through TCF4-mediated GPX4 transcription.
    Article Snippet: 2.1 Antibodies and Reagents Antibodies: β-catenin antibody (ABclonal, China), Pan-Kla antibody (ABclonal, China, Cat. No.: A23004), β-catenin-Kla antibody (custom site-specific antibody, Beinlai Biotechnology Co., Ltd.), GPX4 antibody (ABclonal, China), GAPDH antibody (ABclonal, China), goat anti-rabbit IgG-HRP (ABclonal, China), goat anti-mouse IgGHRP (ABclonal, China). .. Reagents: Fetal bovine serum (FBS, Gibco, USA), DMEM medium (Gibco, USA), RPMI-1640 medium (Gibco, USA), PBS buffer (Kaiji Biology, China), trypsin (Gibco, USA), cryopreservation solution (Saiwen Innovation, China), paraformaldehyde (Solarbio, China), 10×TBST buffer (Solarbio, China), Triton X-100 (Solarbio, China), Trizol reagent (Invitrogen, USA), isopropanol (Sinopharm, China), absolute ethanol (Shanghai Bio-Chemical Co., Ltd., China), TB Green Premix Ex Taq II (Takara, Japan), reverse transcription kit (Takara, Japan), Puromycin dihydrochloride (MCE, USA), PCR primers (Sangon Biotech, China), overexpression virus (Sangon Biotech, China), knockdown virus (Sangon Biotech, China), CCK-8 kit (Solarbio, China), Matrigel Matrix (Corning, USA), EdU cell proliferation assay kit (Solarbio, China), protein AR TIC LE IN PR ES S extraction kit (Kaiji Biology, China), BCA protein assay kit (Beyotime, China), SDSPAGE gel preparation kit (Kaiji Biology, China), PVDF membrane (Millipore, USA), ECL chemiluminescence reagent (Tanon, China), reduced glutathione (GSH) assay kit (Solarbio, China), lipid peroxide (LPO) assay kit (Solarbio, China). lactic acid (LA, Sigma-Aldrich, USA), 2-deoxyglucose (2-DG, Sigma-Aldrich, USA), Fer-1 (Abcam, UK), Beclin 1Bioswamp, China. ..

    Proliferation Assay:

    Article Title: Lactylation of β-catenin promotes non-small cell lung cancer by inhibiting ferroptosis through TCF4-mediated GPX4 transcription.
    Article Snippet: 2.1 Antibodies and Reagents Antibodies: β-catenin antibody (ABclonal, China), Pan-Kla antibody (ABclonal, China, Cat. No.: A23004), β-catenin-Kla antibody (custom site-specific antibody, Beinlai Biotechnology Co., Ltd.), GPX4 antibody (ABclonal, China), GAPDH antibody (ABclonal, China), goat anti-rabbit IgG-HRP (ABclonal, China), goat anti-mouse IgGHRP (ABclonal, China). .. Reagents: Fetal bovine serum (FBS, Gibco, USA), DMEM medium (Gibco, USA), RPMI-1640 medium (Gibco, USA), PBS buffer (Kaiji Biology, China), trypsin (Gibco, USA), cryopreservation solution (Saiwen Innovation, China), paraformaldehyde (Solarbio, China), 10×TBST buffer (Solarbio, China), Triton X-100 (Solarbio, China), Trizol reagent (Invitrogen, USA), isopropanol (Sinopharm, China), absolute ethanol (Shanghai Bio-Chemical Co., Ltd., China), TB Green Premix Ex Taq II (Takara, Japan), reverse transcription kit (Takara, Japan), Puromycin dihydrochloride (MCE, USA), PCR primers (Sangon Biotech, China), overexpression virus (Sangon Biotech, China), knockdown virus (Sangon Biotech, China), CCK-8 kit (Solarbio, China), Matrigel Matrix (Corning, USA), EdU cell proliferation assay kit (Solarbio, China), protein AR TIC LE IN PR ES S extraction kit (Kaiji Biology, China), BCA protein assay kit (Beyotime, China), SDSPAGE gel preparation kit (Kaiji Biology, China), PVDF membrane (Millipore, USA), ECL chemiluminescence reagent (Tanon, China), reduced glutathione (GSH) assay kit (Solarbio, China), lipid peroxide (LPO) assay kit (Solarbio, China). lactic acid (LA, Sigma-Aldrich, USA), 2-deoxyglucose (2-DG, Sigma-Aldrich, USA), Fer-1 (Abcam, UK), Beclin 1Bioswamp, China. ..

    Bicinchoninic Acid Protein Assay:

    Article Title: Lactylation of β-catenin promotes non-small cell lung cancer by inhibiting ferroptosis through TCF4-mediated GPX4 transcription.
    Article Snippet: 2.1 Antibodies and Reagents Antibodies: β-catenin antibody (ABclonal, China), Pan-Kla antibody (ABclonal, China, Cat. No.: A23004), β-catenin-Kla antibody (custom site-specific antibody, Beinlai Biotechnology Co., Ltd.), GPX4 antibody (ABclonal, China), GAPDH antibody (ABclonal, China), goat anti-rabbit IgG-HRP (ABclonal, China), goat anti-mouse IgGHRP (ABclonal, China). .. Reagents: Fetal bovine serum (FBS, Gibco, USA), DMEM medium (Gibco, USA), RPMI-1640 medium (Gibco, USA), PBS buffer (Kaiji Biology, China), trypsin (Gibco, USA), cryopreservation solution (Saiwen Innovation, China), paraformaldehyde (Solarbio, China), 10×TBST buffer (Solarbio, China), Triton X-100 (Solarbio, China), Trizol reagent (Invitrogen, USA), isopropanol (Sinopharm, China), absolute ethanol (Shanghai Bio-Chemical Co., Ltd., China), TB Green Premix Ex Taq II (Takara, Japan), reverse transcription kit (Takara, Japan), Puromycin dihydrochloride (MCE, USA), PCR primers (Sangon Biotech, China), overexpression virus (Sangon Biotech, China), knockdown virus (Sangon Biotech, China), CCK-8 kit (Solarbio, China), Matrigel Matrix (Corning, USA), EdU cell proliferation assay kit (Solarbio, China), protein AR TIC LE IN PR ES S extraction kit (Kaiji Biology, China), BCA protein assay kit (Beyotime, China), SDSPAGE gel preparation kit (Kaiji Biology, China), PVDF membrane (Millipore, USA), ECL chemiluminescence reagent (Tanon, China), reduced glutathione (GSH) assay kit (Solarbio, China), lipid peroxide (LPO) assay kit (Solarbio, China). lactic acid (LA, Sigma-Aldrich, USA), 2-deoxyglucose (2-DG, Sigma-Aldrich, USA), Fer-1 (Abcam, UK), Beclin 1Bioswamp, China. ..

    Membrane:

    Article Title: Lactylation of β-catenin promotes non-small cell lung cancer by inhibiting ferroptosis through TCF4-mediated GPX4 transcription.
    Article Snippet: 2.1 Antibodies and Reagents Antibodies: β-catenin antibody (ABclonal, China), Pan-Kla antibody (ABclonal, China, Cat. No.: A23004), β-catenin-Kla antibody (custom site-specific antibody, Beinlai Biotechnology Co., Ltd.), GPX4 antibody (ABclonal, China), GAPDH antibody (ABclonal, China), goat anti-rabbit IgG-HRP (ABclonal, China), goat anti-mouse IgGHRP (ABclonal, China). .. Reagents: Fetal bovine serum (FBS, Gibco, USA), DMEM medium (Gibco, USA), RPMI-1640 medium (Gibco, USA), PBS buffer (Kaiji Biology, China), trypsin (Gibco, USA), cryopreservation solution (Saiwen Innovation, China), paraformaldehyde (Solarbio, China), 10×TBST buffer (Solarbio, China), Triton X-100 (Solarbio, China), Trizol reagent (Invitrogen, USA), isopropanol (Sinopharm, China), absolute ethanol (Shanghai Bio-Chemical Co., Ltd., China), TB Green Premix Ex Taq II (Takara, Japan), reverse transcription kit (Takara, Japan), Puromycin dihydrochloride (MCE, USA), PCR primers (Sangon Biotech, China), overexpression virus (Sangon Biotech, China), knockdown virus (Sangon Biotech, China), CCK-8 kit (Solarbio, China), Matrigel Matrix (Corning, USA), EdU cell proliferation assay kit (Solarbio, China), protein AR TIC LE IN PR ES S extraction kit (Kaiji Biology, China), BCA protein assay kit (Beyotime, China), SDSPAGE gel preparation kit (Kaiji Biology, China), PVDF membrane (Millipore, USA), ECL chemiluminescence reagent (Tanon, China), reduced glutathione (GSH) assay kit (Solarbio, China), lipid peroxide (LPO) assay kit (Solarbio, China). lactic acid (LA, Sigma-Aldrich, USA), 2-deoxyglucose (2-DG, Sigma-Aldrich, USA), Fer-1 (Abcam, UK), Beclin 1Bioswamp, China. ..

    GSH Assay:

    Article Title: Lactylation of β-catenin promotes non-small cell lung cancer by inhibiting ferroptosis through TCF4-mediated GPX4 transcription.
    Article Snippet: 2.1 Antibodies and Reagents Antibodies: β-catenin antibody (ABclonal, China), Pan-Kla antibody (ABclonal, China, Cat. No.: A23004), β-catenin-Kla antibody (custom site-specific antibody, Beinlai Biotechnology Co., Ltd.), GPX4 antibody (ABclonal, China), GAPDH antibody (ABclonal, China), goat anti-rabbit IgG-HRP (ABclonal, China), goat anti-mouse IgGHRP (ABclonal, China). .. Reagents: Fetal bovine serum (FBS, Gibco, USA), DMEM medium (Gibco, USA), RPMI-1640 medium (Gibco, USA), PBS buffer (Kaiji Biology, China), trypsin (Gibco, USA), cryopreservation solution (Saiwen Innovation, China), paraformaldehyde (Solarbio, China), 10×TBST buffer (Solarbio, China), Triton X-100 (Solarbio, China), Trizol reagent (Invitrogen, USA), isopropanol (Sinopharm, China), absolute ethanol (Shanghai Bio-Chemical Co., Ltd., China), TB Green Premix Ex Taq II (Takara, Japan), reverse transcription kit (Takara, Japan), Puromycin dihydrochloride (MCE, USA), PCR primers (Sangon Biotech, China), overexpression virus (Sangon Biotech, China), knockdown virus (Sangon Biotech, China), CCK-8 kit (Solarbio, China), Matrigel Matrix (Corning, USA), EdU cell proliferation assay kit (Solarbio, China), protein AR TIC LE IN PR ES S extraction kit (Kaiji Biology, China), BCA protein assay kit (Beyotime, China), SDSPAGE gel preparation kit (Kaiji Biology, China), PVDF membrane (Millipore, USA), ECL chemiluminescence reagent (Tanon, China), reduced glutathione (GSH) assay kit (Solarbio, China), lipid peroxide (LPO) assay kit (Solarbio, China). lactic acid (LA, Sigma-Aldrich, USA), 2-deoxyglucose (2-DG, Sigma-Aldrich, USA), Fer-1 (Abcam, UK), Beclin 1Bioswamp, China. ..



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